Autophagy-related genes are potential diagnostic and prognostic biomarkers in prostate cancer

Recently, autophagy was found related to several malignances.

To explore the diagnostic and prognostic values of autophagy in prostate cancer (PCa), we first identified differentially expressed autophagy-related genes (DEARGs) based on The Cancer Genome Atlas (TCGA) Prostate Adenocarcinoma (PRAD) dataset. Gene ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment were applied to perform gene functional annotation. Then, multivariate logistic regression analysis was applied to construct the risk index (RI). Receiver operating characteristic (ROC), calibration and decision curve analysis (DCA) curves were performed to identify the effectiveness of RI. Next, multivariate Cox regression analyses were performed to construct the prognostic index (PI) and autophagy-clinical prognostic index (ACPI).

We identified 16 DEARGs and functional annotation demonstrated the relevance of these genes to autophagy and revealed the association of these DEARGs with digestive system, drug resistance and apoptosis. Then, the RI was constructed based on 5 DEARGs and the area under the ROC curve (AUC) was 0.9858. Validation based on Gene Expression Omnibus (GEO) datasets suggested that the RI was effective. Next, 7 ARGs were identified associated with overall survival (OS) and the PI was developed composed of 3 ARGs. Finally, ACPI was constructed based on PI and the M stage.

This study provided potential models for predicting the risk and prognosis of PCa and indicated the molecular insights of autophagy in PCa. While no other dataset was applied to test the effectiveness of the PI and ACPI models attribute to the well prognosis of PCa.