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Preparation of Mitochondrial Enriched Fractions for Metabolic Analysis in Drosophila
Published on: September 30, 2015
The mitochondrial genome of Leptopeza flavipes (Diptera: Empididae)
Yue Liu1,2, Mengqing Wang3, NaiZhong Chen2
1College of Plant Protection, China Agricultural University, Beijing, China.
Abstract:
The dance fly Leptopeza flavipes belongs to the subfamily Ocydromiinae of Empididae. The mitogenome (GenBank accession number: MT610901) of L. flavipes was sequenced, the new representative of the mitogenome of the subfamily. The nearly complete mitogenome is 15,267 bp totally, consisting of 13 protein-coding genes, 2 rRNAs and 22 transfer RNAs. All genes have similar locations and strands with that of other published species of Empididae. The nucleotide composition biases toward A and T, which together made up 78.1%of the entirety. Bayesian inference analysis strongly supported the monophyly of Empidoidea, Empididae and Dolichopodidae. It is clear that the phylogenetic relationship within Empidoidea: (Dolichopodinae + Neurigoninae) + ((Empidinae + (Trichopezinae + Oreogetoninae)) + Ocydromiinae) in this study.
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