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Updated: Nov 21, 2025

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
Published on: July 28, 2017
The complete chloroplast genome of Ulmus parvifolia, an important landscaping tree
Yunzhou Lyu1, Min Zhai2,3, Zeping Jiang1
1Jiangsu Academy of Forestry, Nanjing, China.
Abstract:
Ulmus parvifolia is a promising tree species for landscaping. In this study, the complete genome of U. parvifolia was reported using next-generation sequencing technology. The chloroplast genome was a circular double-stranded DNA molecule with 159,182 bp in length. It contained a large single copy (LSC) region of 87,838 bp, a small single copy (SSC) region of 18,750 bp, and two inverted repeat (IRa and IRb) regions of 26,297 bp each, which exhibited a typical quadripartite structure. A total of 133 genes were identified, including 84 protein-coding genes, 41 tRNA genes, and eight rRNA genes. The overall GC content in the chloroplast genome was 35.59%. Phylogenetic analysis indicated that U. parvifolia, as a representative of Sect. Microptelea within the Ulmus genus, is sister to the species of Sect. Ulmus.
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