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Do DNA polymerases delta and alpha act coordinately as leading and lagging strand replicases?
F Focher1, E Ferrari, S Spadari
1Department of Pharmacology and Biochemistry, University of Zürich-Irchel, Switzerland.
FEBS Letters
|February 29, 1988
Abstract:
The activity ratio of DNA polymerases delta and alpha in calf thymus was found to be invariably 1:1, irrespective of extraction procedure (8 types) and subcellular localization (cytoplasm, nucleus and microsomes). This was established by separation of the two forms by hydroxyapatite chromatography and by their response to specific inhibitors and monoclonal antibodies. This finding supports the dimeric DNA polymerase model [(1980) J. Biol. Chem. 255, 4290-4303], which proposes that DNA polymerases delta and alpha act coordinately as leading and lagging strand enzymes, respectively, at the replication fork.