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Psoralen photofootprinting of protein-binding sites on DNA
W P Zhen1, C Jeppesen, P E Nielsen
1Department of Biochemistry B, Panum Institute, University of Copenhagen, Denmark.
FEBS Letters
|February 29, 1988
Abstract:
Using a BAL31 exonuclease assay to determine the sites of 4,5',8-trimethylpsoralen photocrosslinking in DNA we have shown that 5'-TA sites which are accessible to psoralen DNA interstrand photocrosslinking in naked DNA become inaccessible when protein, in casu, lambda-repressor E. coli or RNA polymerase are bound at their recognition DNA sequences (OR1 operator or deo1 promoter, respectively). These results show that psoralens can be used as photofootprinting reagents to study specific protein-DNA interactions.