Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

One-Compartment Open Model: Wagner-Nelson and Loo Riegelman Method for ka Estimation01:24

One-Compartment Open Model: Wagner-Nelson and Loo Riegelman Method for ka Estimation

916
This lesson introduces two critical methods in pharmacokinetics, the Wagner-Nelson and Loo-Riegelman methods, used for estimating the absorption rate constant (ka) for drugs administered via non-intravenous routes. The Wagner-Nelson method relates ka to the plasma concentration derived from the slope of a semilog percent unabsorbed time plot. However, it is limited to drugs with one-compartment kinetics and can be impacted by factors like gastrointestinal motility or enzymatic degradation.
On...
916
Differential Staining Technique01:26

Differential Staining Technique

1.4K
Differential staining is an essential microbiological technique that exploits variations in cell wall structures to classify and identify microorganisms. It facilitates the distinction of bacteria, aiding in diagnostic and research applications. Two of the most widely used differential staining methods are Gram staining and acid-fast staining, both of which rely on the chemical and structural differences in bacterial cell walls.Gram Staining TechniqueGram staining differentiates bacteria by...
1.4K
Extraction: Partition and Distribution Coefficients01:14

Extraction: Partition and Distribution Coefficients

4.1K
The distribution law or Nernst's distribution law is the law that governs the distribution of a solute between two immiscible solvents. This law, also known as the partition law, states that if a solute is added to the mixture of two immiscible solvents at a constant temperature, the solute is distributed between the two solvents in such a way that the ratio of solute concentrations in the solvents remains constant at equilibrium.
For extracting a solute from an aqueous phase into an...
4.1K
Simple Staining Technique01:24

Simple Staining Technique

1.9K
OverviewStaining techniques in microscopy enhance the visualization of microorganisms by increasing contrast and allowing the differentiation of cellular structures. Simple staining is one of the fundamental methods used to observe the basic morphological characteristics of microorganisms, including their size, shape, and arrangement. This method relies on the application of a single dye to stain the entire cell, producing a clear contrast between the cell and the background.FixationFixation is...
1.9K
Special Staining Techniques01:13

Special Staining Techniques

864
Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...
864
Wald-Wolfowitz Runs Test II01:17

Wald-Wolfowitz Runs Test II

398
The Wald-Wolfowitz runs test, commonly referred to as the runs test, is a nonparametric test used to assess the randomness of ordered data. The test evaluates the number of runs, which are consecutive sequences of similar elements within the data. If the number of runs is significantly higher or lower than expected, the data is considered non-random, indicating a detectable pattern or structure.
For binary data, runs are identified using symbols such as + and −, or equivalently, 1s and 0s. In...
398

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

RT-GAN: Recurrent Temporal GAN for Adding Lightweight Temporal Consistency to Frame-Based Domain Translation Approaches.

Medical image computing and computer-assisted intervention : MICCAI ... International Conference on Medical Image Computing and Computer-Assisted Intervention·2026
Same author

Recycling and Environmental Sustainability in Anesthesia Practice: Beyond Low‑Flow Anesthesia.

Cureus·2026
Same author

Tumor Microenvironment: Insights from Multiparametric MRI in Pancreatic Ductal Adenocarcinoma.

Cancers·2026
Same author

Whole Slide Imaging in Genitourinary Pathology:<br /> A Cloud-Based Digitisation Workflow for Resource-Limited Settings.

Journal of the College of Physicians and Surgeons--Pakistan : JCPSP·2025
Same author

Healthcare Utilization Unchanged in the Control Arm of a Randomized Clinical Trial.

Journal of primary care & community health·2025
Same author

Efficacy and Safety of Erector Spinae Plane Block in Pain Management for Inguinal Hernia Repair: An Updated Systematic Review and Meta-Analysis of Randomized Controlled Trials.

World journal of surgery·2025

Related Experiment Video

Updated: Nov 21, 2025

A Multimodal Imaging Framework to Advance Phenotyping of Living Label-free Breast Cancer Cells
10:37

A Multimodal Imaging Framework to Advance Phenotyping of Living Label-free Breast Cancer Cells

Published on: August 22, 2025

799

Multimarginal Wasserstein Barycenter for Stain Normalization and Augmentation.

Saad Nadeem1, Travis Hollmann2, Allen Tannenbaum3

  • 1Department of Medical Physics, Memorial Sloan Kettering Cancer Center.

Medical Image Computing and Computer-Assisted Intervention : MICCAI ... International Conference on Medical Image Computing and Computer-Assisted Intervention
|January 18, 2021
PubMed
Summary

This study introduces a novel method using multimarginal Wasserstein barycenter to normalize and augment hematoxylin and eosin (H&E) stained images, improving diagnostic accuracy. The approach enhances stain consistency and boosts performance in nuclei segmentation tasks.

Keywords:
Stain NormalizationWasserstein Barycenter

More Related Videos

Applying Hyperspectral Reflectance Imaging to Investigate the Palettes and the Techniques of Painters
07:05

Applying Hyperspectral Reflectance Imaging to Investigate the Palettes and the Techniques of Painters

Published on: June 18, 2021

2.6K
Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
14:23

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

Published on: March 6, 2018

11.1K

Related Experiment Videos

Last Updated: Nov 21, 2025

A Multimodal Imaging Framework to Advance Phenotyping of Living Label-free Breast Cancer Cells
10:37

A Multimodal Imaging Framework to Advance Phenotyping of Living Label-free Breast Cancer Cells

Published on: August 22, 2025

799
Applying Hyperspectral Reflectance Imaging to Investigate the Palettes and the Techniques of Painters
07:05

Applying Hyperspectral Reflectance Imaging to Investigate the Palettes and the Techniques of Painters

Published on: June 18, 2021

2.6K
Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
14:23

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

Published on: March 6, 2018

11.1K

Area of Science:

  • Digital Pathology
  • Computational Imaging
  • Medical Image Analysis

Background:

  • Variations in hematoxylin and eosin (H&E) staining impact clinical diagnosis accuracy.
  • Standardizing H&E images is crucial for reliable diagnostic outcomes.

Purpose of the Study:

  • To develop a robust method for normalizing and augmenting H&E stained images.
  • To address variations caused by clinical lab protocols and scanners.

Main Methods:

  • Utilized multimarginal Wasserstein barycenter for stain normalization and augmentation.
  • Incorporated intermediate reference images for simultaneous normalization and augmentation.
  • Employed a quantitative and qualitative evaluation framework.

Main Results:

  • The proposed approach demonstrated superior performance compared to existing stain normalization methods.
  • Achieved state-of-the-art results in nuclei segmentation tasks post-normalization and augmentation.

Conclusions:

  • The multimarginal Wasserstein barycenter offers a mathematically robust solution for H&E image standardization.
  • This method significantly improves the reliability of digital pathology diagnostics and downstream analysis.