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Measurement of Lung Vessel and Epithelial Permeability In Vivo with Evans Blue
Prestina Smith1, Lauren A Jeffers1, Michael Koval2,3
1Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, Department of Medicine, Emory University School of Medicine, Atlanta, GA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|January 18, 2021
Abstract:
Lung fluid balance is maintained in part by the barriers formed by the pulmonary microvasculature and alveolar epithelium. Failure of either of these barriers leads to pulmonary edema, which limits lung function and exacerbates the severity of acute lung injury. Here we describe a method using Evans Blue dye to simultaneously measure the function of vascular and epithelial barriers of murine lungs in vivo.
