Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Leaky Scanning02:28

Leaky Scanning

5.4K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.4K
Viral Structure00:56

Viral Structure

70.8K
Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
70.8K
Conjugated Proteins02:50

Conjugated Proteins

24.1K
Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
24.1K
Protein Folding01:22

Protein Folding

124.6K
Overview
124.6K
Protein Folding Quality Check in the RER01:29

Protein Folding Quality Check in the RER

4.7K
ER is the primary site for the maturation and folding of soluble and transmembrane secretory proteins. The calnexin cycle is a specific chaperone system that folds and assesses the confirmation of N-glycosylated proteins before they can exit the ER lumen. The primary players of this quality check pipeline are the lectins, ER-resident chaperones, and a glucosyl transferase enzyme. In case the calnexin system in the lumen fails to salvage a misfolded protein, it is transported to the cytoplasm...
4.7K
RNA Structure01:23

RNA Structure

77.4K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
77.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Privateer for UCSF ChimeraX: Carbohydrate structure validation and visualization.

Protein science : a publication of the Protein Society·2026
Same author

Allosteric disordering of eIF2B regulates the integrated stress response.

Nature chemical biology·2026
Same author

Pearls and Pitfalls of Ponseti Method Bracing for Idiopathic Clubfoot-An Institutional Guide to Optimize Orthotic Management.

Journal of the Pediatric Orthopaedic Society of North America·2026
Same author

A mentorship model for robotic-assisted navigation in pediatric spine surgery.

Journal of robotic surgery·2026
Same author

Evaluation of Commonly Utilized Artificial Intelligence Large Language Models in Optimizing Readability, Accuracy, and Comprehension of Orthopaedic Oncology Patient Educational Materials.

The Journal of the American Academy of Orthopaedic Surgeons·2026
Same author

Trends of E-Scooter-Related Pediatric Orthopaedic Injuries: 20 Years of Nationally Representative Data.

The Journal of the American Academy of Orthopaedic Surgeons·2026

Related Experiment Video

Updated: Nov 21, 2025

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy
07:56

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

Published on: July 29, 2021

3.8K

Improving SARS-CoV-2 structures: Peer review by early coordinate release.

Tristan I Croll1, Christopher J Williams2, Vincent B Chen2

  • 1CIMR, University of Cambridge, Cambridge, United Kingdom.

Biophysical Journal
|January 18, 2021
PubMed
Summary
This summary is machine-generated.

Researchers identified and corrected errors in early severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) protein structures, improving accuracy for vaccine and drug development. These corrections enhance the reliability of crucial structural data for the scientific community.

More Related Videos

Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit
22:10

Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit

Published on: June 28, 2013

13.5K
Production of a SARS-CoV-2 Virus-Like-Particle System to Investigate Viral Life Cycles In Vitro
09:26

Production of a SARS-CoV-2 Virus-Like-Particle System to Investigate Viral Life Cycles In Vitro

Published on: June 6, 2025

844

Related Experiment Videos

Last Updated: Nov 21, 2025

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy
07:56

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

Published on: July 29, 2021

3.8K
Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit
22:10

Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit

Published on: June 28, 2013

13.5K
Production of a SARS-CoV-2 Virus-Like-Particle System to Investigate Viral Life Cycles In Vitro
09:26

Production of a SARS-CoV-2 Virus-Like-Particle System to Investigate Viral Life Cycles In Vitro

Published on: June 6, 2025

844

Area of Science:

  • Structural Biology
  • Biophysics
  • Computational Biology

Background:

  • Rapid release of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) structures is vital for vaccine and drug development.
  • Ensuring the accuracy of these experimental three-dimensional structures is critical for reliable downstream applications.
  • Occasional errors in structural data can have significant implications for research and therapeutic strategies.

Purpose of the Study:

  • To systematically survey early SARS-CoV-2 protein structures for errors.
  • To provide higher-accuracy corrections for identified structural inaccuracies.
  • To establish a rigorous and efficient method for structural data validation and refinement.

Main Methods:

  • Utilized novel validation and correction tools, including CaBLAM and ISOLDE.
  • Performed detailed analysis of seven early SARS-CoV-2 structures.
  • Communicated findings and corrections to depositors via documented reports.

Main Results:

  • Identified and corrected significant errors in five SARS-CoV-2 structures.
  • Corrections included sequence misalignments, incorrect RNA conformations, and peptide flips.
  • Proposed changes were validated by subsequent higher-resolution data for two structures.
  • Improvements propagated to subsequently determined related structures.

Conclusions:

  • New validation methods can rapidly identify and correct critical errors in protein structural data.
  • Direct communication with depositors facilitates timely updates to the Protein Data Bank (PDB).
  • This rigorous, coordinate-based review process offers a faster and stricter alternative to traditional peer review.