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Generating a fucose permease deletion mutant in Bifidobacterium longum subspecies infantis ATCC 15697
Melanie A Higgins1, Katherine S Ryan1
1Department of Chemistry, The University of British Columbia, Vancouver, British Columbia, V6T 1Z1, Canada.
Anaerobe
|January 18, 2021
Abstract:
Bifidobacterium longum subsp. infantis ATCC 15697 has emerged as a model for infant gut-associated bifidobacterial strains. Here we present a genetic system for B. longum subsp. infantis ATCC 15697 using its own DNA restriction-modification systems and create a fucose permease deletion mutant lacking the ability to use free fucose as a carbon source.
