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Updated: Nov 21, 2025
Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes
Published on: June 5, 2017
Characterization of cell type-specific S100B expression in the mouse olfactory bulb.
Xin Su1, Tamara Vasilkovska1, Nicole Fröhlich1
1Institute of Physiology, Department of Neurophysiology, Eberhard Karls University of Tübingen, Tübingen, Germany.
S100B protein is found in astrocytes and myelinating oligodendrocytes within the mouse olfactory bulb, playing a role in oligodendrocyte density and myelin sheath maintenance.
Area of Science:
- Neuroscience
- Cell Biology
- Biochemistry
Background:
- S100B is a calcium-binding protein involved in neurogenesis and brain immune system interactions.
- The olfactory bulb is a key neurogenic niche containing diverse cell types.
Purpose of the Study:
- To characterize S100B expression in the mouse olfactory bulb.
- To identify which cell types express S100B in this region.
Main Methods:
- Immunohistochemistry to detect S100B expression.
- Analysis of S100B localization in different olfactory bulb layers and cell types.
Main Results:
- S100B is expressed in astrocytes, NG2 cells, and APC-positive myelinating oligodendrocytes.
- S100B is not found in mature/adult-born neurons or microglia.
- Oligodendrocyte density correlates with S100B-positive myelinating oligodendrocytes.
Conclusions:
- S100B is a marker for myelinating oligodendrocytes in the olfactory bulb.
- S100B is important for the establishment and maintenance of myelin sheaths.
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