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Related Concept Videos

Oxygen Delivering System I: Nasal Cannula and Face Mask01:26

Oxygen Delivering System I: Nasal Cannula and Face Mask

The human body requires oxygen to function, and when the natural process of respiration is hindered, external devices, including the following, are needed to help deliver this vital gas.
Nasal Cannula
A nasal cannula is a lightweight tube split at one end into two prongs and placed in the nostrils. It is typically used to deliver low to medium levels of oxygen.
Suggested flow rate: The suggested flow rate for a nasal cannula typically ranges between 1 and 6 L/min.
Oxygen percentage setting:...
Oxygen Delivering System II: Venturi Mask and Transtracheal Oxygen01:16

Oxygen Delivering System II: Venturi Mask and Transtracheal Oxygen

Oxygen therapy is a pivotal aspect of medical care, particularly for patients with respiratory ailments. Two prominent oxygen-delivering systems include the Venturi mask and the transtracheal oxygen catheter.
Venturi Mask
The Venturi mask, named after the Venturi effect, is designed to deliver precise oxygen concentrations. It consists of a large tube with an oxygen inlet that narrows down, causing a pressure drop that pulls air in through adjustable side ports. The mask is a lightweight,...
Oxygen Delivering System III: Tracheostomy and T-piece01:23

Oxygen Delivering System III: Tracheostomy and T-piece

Oxygen delivery is critical in clinical care, especially for patients with respiratory disorders or those undergoing surgical procedures. Various systems, such as tracheostomy and the T-piece, deliver oxygen to the lungs, ensuring adequate arterial oxygenation.
Tracheostomy
A tracheostomy is a surgically created opening (stoma) in the anterior part of the trachea. It is used to establish a patient airway, bypass an upper airway obstruction, simplify the removal of secretions, permit long-term...
Administering Oxygen by Mask01:30

Administering Oxygen by Mask

Administering Oxygen by Mask
Administering oxygen by mask is a common nursing intervention that provides supplemental oxygen to patients with respiratory distress or chronic lung conditions. This procedure involves delivering oxygen at a specified rate through a face mask connected to an oxygen source.
Equipment
The equipment necessary for this procedure includes:
Bioreactor Controls-II01:18

Bioreactor Controls-II

In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...
Bioreactor Controls-III01:22

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...

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Related Experiment Video

Updated: Jun 27, 2026

Fabrication and Operation of an Oxygen Insert for Adherent Cellular Cultures
11:56

Fabrication and Operation of an Oxygen Insert for Adherent Cellular Cultures

Published on: January 6, 2010

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96-Well Oxygen Control Using a 3D-Printed Device.

Adam Szmelter1, Jason Jacob1, David T Eddington1

  • 1Department of Bioengineering, University of Illinois at Chicago, 851 South Morgan Street, Chicago, Illinois 60607, United States.

Analytical Chemistry
|January 19, 2021
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel device for high-throughput oxygen studies. This 96-well plate system precisely controls oxygen levels, enabling detailed analysis of cell responses to varying oxygen concentrations and drug treatments.

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Quantitative and Temporal Control of Oxygen Microenvironment at the Single Islet Level
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Last Updated: Jun 27, 2026

Fabrication and Operation of an Oxygen Insert for Adherent Cellular Cultures
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Creating Rapid Oxygen Oscillations in Microbial Single-cell Growth Analysis using a Microfluidic Double-layer Device
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Creating Rapid Oxygen Oscillations in Microbial Single-cell Growth Analysis using a Microfluidic Double-layer Device

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327

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Physiology

Background:

  • Oxygen concentration is critical for cellular processes like differentiation and drug metabolism.
  • Current methods use low-throughput incubators or hypoxia chambers, limiting simultaneous experiments.
  • A need exists for higher-throughput methods to study oxygen's biological roles.

Purpose of the Study:

  • To introduce a device for delivering 12 unique oxygen concentrations to a 96-well plate.
  • To enable seamless integration into existing biomedical research workflows.
  • To facilitate high-throughput studies on oxygen's effect on cell behavior and drug response.

Main Methods:

  • A device was designed to deliver controlled oxygen concentrations to the headspace of each well in a 96-well plate.
  • Gas-tight gaskets created 96 individual "mini-incubators" within the plate.
  • A gas-mixing gradient generator and flow-splitting network supplied 12 distinct oxygen levels (0.6-20.5%).

Main Results:

  • The device successfully generated a range of oxygen concentrations across the 96-well plate.
  • A549 lung carcinoma cells showed a stepwise increase in death below 9% oxygen when treated with tirapirazamine (TPZ).
  • A 3D dose-response landscape was generated by testing multiple TPZ concentrations across the oxygen gradient.

Conclusions:

  • Microfluidic technology can be integrated with standard labware for enhanced oxygen studies.
  • The developed device significantly increases throughput for investigating oxygen's role in biological systems.
  • This approach allows for more comprehensive analysis of cellular responses under precisely controlled oxygen conditions.