Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Postpartum haemorrhage: epidemiology, consequences, and missed opportunities.

Lancet (London, England)·2026
Same author

The effects of predischarge postnatal care interventions following institutional deliveries in India on maternal and newborn health outcomes in the first year after discharge: A systematic review of quantitative and qualitative studies.

Journal of family medicine and primary care·2026
Same author

DPP-Mediated Interaction of TAZ/β-Catenin Promotes the Differentiation of DPSCs into Odontoblasts.

International journal of molecular sciences·2026
Same author

Response to comment on "Perioperative copeptin elevations and prediction of myocardial injury and acute kidney injury after abdominal surgery: A preliminary prospective observational study".

Journal of the Formosan Medical Association = Taiwan yi zhi·2026
Same author

A role for ETV1 and endothelial cell-derived extracellular vesicle microRNAs in priming fibroblast response to vesicle-bound FGF2.

bioRxiv : the preprint server for biology·2026
Same author

Multi-omics analysis of type II diabetic wound healing reveals CD44-mediated immune cell crosstalk dysfunction in mice and humans.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Nov 20, 2025

Author Spotlight: Improving the Production of Self-Assembling Fibers and Peptide Hydrogels for Superior Biocompatibility
05:24

Author Spotlight: Improving the Production of Self-Assembling Fibers and Peptide Hydrogels for Superior Biocompatibility

Published on: September 6, 2024

1.5K

Engineering a Self-Assembling Leucine Zipper Hydrogel System with Function-Specific Motifs for Tissue Regeneration.

Chun-Chieh Huang1, Sriram Ravindran1, Miya Kang1

  • 1Brodie Tooth Development Genetics & Regenerative Medicine Research Laboratory Department of Oral Biology, University of Illinois at Chicago, 801 South Paulina Street, Room 561C, Chicago, Illinois 60612, United States.

ACS Biomaterials Science & Engineering
|January 19, 2021
PubMed
Summary

This study developed a tunable, protein-based hydrogel scaffold for tissue regeneration. The leucine zipper (LZ) hydrogel enhanced bone regeneration by controlling growth factor release for stem cell therapy.

Keywords:
biomimetic peptidesbone regenerationgrowth factor deliverymesenchymal stem cellsosteogenic differentiationself-assembling scaffolds

More Related Videos

The Synthesis of RGD-functionalized Hydrogels as a Tool for Therapeutic Applications
09:30

The Synthesis of RGD-functionalized Hydrogels as a Tool for Therapeutic Applications

Published on: October 7, 2016

11.6K
Engineering a Bilayered Hydrogel to Control ASC Differentiation
07:48

Engineering a Bilayered Hydrogel to Control ASC Differentiation

Published on: May 25, 2012

14.2K

Related Experiment Videos

Last Updated: Nov 20, 2025

Author Spotlight: Improving the Production of Self-Assembling Fibers and Peptide Hydrogels for Superior Biocompatibility
05:24

Author Spotlight: Improving the Production of Self-Assembling Fibers and Peptide Hydrogels for Superior Biocompatibility

Published on: September 6, 2024

1.5K
The Synthesis of RGD-functionalized Hydrogels as a Tool for Therapeutic Applications
09:30

The Synthesis of RGD-functionalized Hydrogels as a Tool for Therapeutic Applications

Published on: October 7, 2016

11.6K
Engineering a Bilayered Hydrogel to Control ASC Differentiation
07:48

Engineering a Bilayered Hydrogel to Control ASC Differentiation

Published on: May 25, 2012

14.2K

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Protein Engineering

Background:

  • Protein-based self-assembling hydrogels offer tunable properties for regenerative medicine.
  • Existing scaffolds often lack specificity for tissue-targeted regeneration.

Purpose of the Study:

  • To develop a leucine zipper (LZ) based self-assembling hydrogel with function-specific motifs for tissue-specific regeneration.
  • To incorporate calcium-binding domains and a heparin-binding domain with a matrix metalloprotease 2 (MMP2) cleavage site for controlled growth factor release.

Main Methods:

  • Engineered LZ hydrogel incorporating dentin matrix protein 1 (DMP1) derived calcium-binding domains (ESQES, QESQSEQS).
  • Incorporated a heparin-binding domain with an MMP2 cleavage site for growth factor (BMP-2, VEGF, TGF-β1) tethering and release.
  • In vitro and in vivo characterization and evaluation of hydrogel scaffolds, including assessment of osteogenic differentiation of human mesenchymal stem cells (HMSCs).

Main Results:

  • The engineered LZ hydrogel demonstrated potential for osteogenic differentiation of stem cells in vivo.
  • A premineralized LZ scaffold loaded with HMSCs showed enhanced osteoinductive properties compared to non-mineralized controls.
  • The LZ hydrogel facilitated controlled release of heparin-binding growth factors (VEGF, TGF-β1, BMP2) in vitro and in vivo, with demonstrated growth factor-specific activity (BMP-2, TGF-β1).

Conclusions:

  • A versatile, protein-based self-assembling hydrogel system with tunable properties was developed.
  • The engineered hydrogel shows promise as a scaffold for tissue regeneration, particularly for osteogenic applications.
  • The system enables controlled delivery and release of specific growth factors, enhancing therapeutic efficacy.