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Human-Derived Scaffold Components and Stem Cells Creating Immunocompatible Dermal Tissue Ensuing Regulated

Rashmi Ramakrishnan1, Harikrishnan V Sreelatha2, Arya Anil2

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ACS Biomaterials Science & Engineering
|January 19, 2021
PubMed
Summary

This study developed a human-derived wound scaffold (AMFIBHA) from amniotic membrane, fibrin, and hyaluronic acid. The AMFIBHA scaffold effectively regenerated large burn wounds, promoting skin appendage formation and reducing immune response.

Keywords:
decellularized amniondermal constructfibrinhyaluronic acidskin tissue engineeringwound care

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Large-sized acute and chronic wounds from burns and diabetes pose significant global health challenges.
  • There is a critical need for immunocompatible matrices for guided wound regeneration, particularly for nonhealing chronic wounds.

Purpose of the Study:

  • To develop a completely human-derived, minimally immune-responsive scaffold (AMFIBHA) for guided wound regeneration.
  • To validate the in vivo potential of the AMFIBHA scaffold for skin regeneration in a burn wound model.

Main Methods:

  • Developed a scaffold (AMFIBHA) combining acellular amniotic membrane (AM), fibrin (FIB), and hyaluronic acid (HA).
  • Standardized decellularization of amnion (dAM) and plasma sterilization (pdAM) for immuno-compatibility and stability.
  • Validated in vitro dermal tissue assembly using human fibroblasts derived from adipose tissue-derived mesenchymal stem cells (hADMSCs).
  • Assessed biocompatibility and in vivo regeneration in a large-sized, full-thickness third-degree burn wound model.

Main Results:

  • The AMFIBHA scaffold demonstrated hemo-/cytocompatibility and supported fibroblast growth, collagen, elastin, and GAG deposition in vitro.
  • Cultured fibroblasts exhibited regulated growth factor expression (TGF-β1, TGF-β3), suggesting a non-fibrotic phenotype.
  • In vivo studies showed reduced immune response for AMFIBHA compared to cellular AM.
  • Complete epithelialization, angiogenesis, and skin appendage development were observed in burn wounds treated with AMFIBHA within 28 days.

Conclusions:

  • The human-derived AMFIBHA scaffold is a promising biomaterial for guided regeneration of large-sized wounds.
  • AMFIBHA demonstrates excellent biocompatibility and promotes effective skin regeneration with reduced fibrosis.
  • This scaffold addresses the unmet clinical need for advanced wound healing solutions for severe burns and chronic wounds.