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Human-Derived Scaffold Components and Stem Cells Creating Immunocompatible Dermal Tissue Ensuing Regulated
Rashmi Ramakrishnan1, Harikrishnan V Sreelatha2, Arya Anil2
1Division of Thrombosis Research, Department of Applied Biology, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology (SCTIMST), Poojappura, Thiruvananthapuram 695012, Kerala, India.
ACS Biomaterials Science & Engineering
|January 19, 2021
Summary
This study developed a human-derived wound scaffold (AMFIBHA) from amniotic membrane, fibrin, and hyaluronic acid. The AMFIBHA scaffold effectively regenerated large burn wounds, promoting skin appendage formation and reducing immune response.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Tissue Engineering
Background:
- Large-sized acute and chronic wounds from burns and diabetes pose significant global health challenges.
- There is a critical need for immunocompatible matrices for guided wound regeneration, particularly for nonhealing chronic wounds.
Purpose of the Study:
- To develop a completely human-derived, minimally immune-responsive scaffold (AMFIBHA) for guided wound regeneration.
- To validate the in vivo potential of the AMFIBHA scaffold for skin regeneration in a burn wound model.
Main Methods:
- Developed a scaffold (AMFIBHA) combining acellular amniotic membrane (AM), fibrin (FIB), and hyaluronic acid (HA).
- Standardized decellularization of amnion (dAM) and plasma sterilization (pdAM) for immuno-compatibility and stability.
- Validated in vitro dermal tissue assembly using human fibroblasts derived from adipose tissue-derived mesenchymal stem cells (hADMSCs).
- Assessed biocompatibility and in vivo regeneration in a large-sized, full-thickness third-degree burn wound model.
Main Results:
- The AMFIBHA scaffold demonstrated hemo-/cytocompatibility and supported fibroblast growth, collagen, elastin, and GAG deposition in vitro.
- Cultured fibroblasts exhibited regulated growth factor expression (TGF-β1, TGF-β3), suggesting a non-fibrotic phenotype.
- In vivo studies showed reduced immune response for AMFIBHA compared to cellular AM.
- Complete epithelialization, angiogenesis, and skin appendage development were observed in burn wounds treated with AMFIBHA within 28 days.
Conclusions:
- The human-derived AMFIBHA scaffold is a promising biomaterial for guided regeneration of large-sized wounds.
- AMFIBHA demonstrates excellent biocompatibility and promotes effective skin regeneration with reduced fibrosis.
- This scaffold addresses the unmet clinical need for advanced wound healing solutions for severe burns and chronic wounds.
