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Supported Membrane Platform to Assess Surface Interactions between Extracellular Vesicles and Stromal Cells
Johana Uribe1, Han-Yuan Liu2, Zeinab Mohamed1
1Meinig School of Biomedical Engineering, Cornell University, 101 Weill Hall, 273 Tower Road, Ithaca, New York 14853, United States.
ACS Biomaterials Science & Engineering
|January 19, 2021
Summary
Researchers developed a novel extracellular vesicle supported bilayer (EVSB) model to study how cancer cell communication with stem cells affects disease. This model revealed that breast cancer EVs enhance stem cell growth and promote conditions favoring cancer progression.
Area of Science:
- Biomaterials Science
- Cell Biology
- Cancer Research
Background:
- Extracellular vesicles (EVs) mediate cell-to-cell communication and cargo exchange.
- EV interactions with stromal cells can influence disease progression, but mechanisms remain unclear.
- Existing models for studying EV-stromal cell surface interactions are limited.
Purpose of the Study:
- To develop a novel model system, the extracellular vesicle supported bilayer (EVSB), for investigating EV-stromal cell surface interactions.
- To analyze the effects of breast cancer EVs on adipose-derived stem cells (ADSCs) using the EVSB model.
- To understand the mechanisms underlying EV-induced cellular changes relevant to disease progression.
Main Methods:
- Creation of an extracellular vesicle supported bilayer (EVSB) incorporating EV membrane material.
- Culturing adipose-derived stem cells (ADSCs) on EVSBs.
- Analysis of ADSC adhesion, spreading, viability, vascular endothelial growth factor (VEGF) secretion, and myofibroblast differentiation.
Main Results:
- ADSCs cultured on EVSBs exhibited enhanced viability, adhesion, and spreading.
- Proangiogenic activity, indicated by increased VEGF secretion, was promoted in ADSCs.
- EV-induced myofibroblast differentiation of ADSCs was not observed.
Conclusions:
- The EVSB model effectively simulates EV-stromal cell surface interactions and their impact on cell behavior.
- Breast cancer EVs promote conditions that enhance oncogenic activity by modulating ADSCs.
- This model system offers a versatile platform for studying EV-mediated cellular changes in various diseases beyond cancer.
