Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

6.1K
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
6.1K
Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

6.1K
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
6.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Towards Reliable Tracking of Natural Killer Cells Using Commercial Iron Oxide Nanoparticles and Magnetic Particle Imaging.

bioRxiv : the preprint server for biology·2026
Same author

Evaluating Intracellular Location and ROS Scavenging by Manganese Dioxide Nanoparticles in Chondrocytes.

ACS applied materials & interfaces·2025
Same author

Isomeric Differences in Nanoparticle's Surface Chemistry Alter Macrophage Interactions In Vitro Due to Protein Corona.

ACS omega·2025
Same author

Optimizing Oxygen-Production Kinetics of Manganese Dioxide Nanoparticles Improves Hypoxia Reversal and Survival in Mice with Bone Metastases.

Molecular pharmaceutics·2024
Same author

Vascular bifurcation influences the protein corona composition on nanoparticles and impacts their cellular uptake.

Nanoscale advances·2022
Same author

PRDX-1 Supports the Survival and Antitumor Activity of Primary and CAR-Modified NK Cells under Oxidative Stress.

Cancer immunology research·2021

Related Experiment Video

Updated: Nov 20, 2025

Measurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells
06:55

Measurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells

Published on: February 22, 2020

19.1K

Engineered Three-Dimensional Tumor Models to Study Natural Killer Cell Suppression.

Madison N Temples1, Isaac M Adjei1, Phoebe M Nimocks1

  • 1J. Crayton Pruitt Department of Biomedical Engineering, University of Florida, Biomedical Sciences Building JG-56, 1275 Center Drive, Gainesville, Florida 32611-6131, United States.

ACS Biomaterials Science & Engineering
|January 19, 2021
PubMed
Summary
This summary is machine-generated.

This study developed a 3D biomaterial to investigate natural killer (NK) cell infiltration in solid tumors. The system revealed that TGF-β inhibition enhances NK cell migration, overcoming tumor microenvironment barriers.

Keywords:
PEG hydrogelimmunosuppressionmigrationnatural killer cellstumor models

More Related Videos

Quantifying Antibody-Dependent Cellular Cytotoxicity in a Tumor Spheroid Model: Application for Drug Discovery
13:19

Quantifying Antibody-Dependent Cellular Cytotoxicity in a Tumor Spheroid Model: Application for Drug Discovery

Published on: April 26, 2024

3.4K
Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors
08:32

Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors

Published on: June 7, 2018

9.9K

Related Experiment Videos

Last Updated: Nov 20, 2025

Measurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells
06:55

Measurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells

Published on: February 22, 2020

19.1K
Quantifying Antibody-Dependent Cellular Cytotoxicity in a Tumor Spheroid Model: Application for Drug Discovery
13:19

Quantifying Antibody-Dependent Cellular Cytotoxicity in a Tumor Spheroid Model: Application for Drug Discovery

Published on: April 26, 2024

3.4K
Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors
08:32

Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors

Published on: June 7, 2018

9.9K

Area of Science:

  • Biomaterials Science
  • Immunology
  • Cancer Research

Background:

  • Natural killer (NK) cell immunotherapies face challenges with infiltration and function within solid tumor microenvironments.
  • Three-dimensional (3D) culture systems are crucial for understanding biophysical and biochemical factors influencing NK cell migration in tumors.

Purpose of the Study:

  • To create a biomaterial supporting NK cell migration and mimicking the in vivo solid tumor microenvironment for studying NK cell infiltration and function.
  • To investigate the impact of biomaterial properties and tumor cell type on NK cell migration and activity in a 3D model.

Main Methods:

  • Development of peptide-functionalized poly(ethylene glycol)-based hydrogels.
  • Encapsulation of lung cancer cells (A549 and H1299) within hydrogels to create 3D tumor microenvironments.
  • Assessment of NK-92 cell migration, cytokine/chemokine production, and cytotoxicity in response to varying biomaterial properties and cancer cell types.
  • Investigating the role of TGF-β signaling by inhibiting its receptor in NK-92 cells.

Main Results:

  • NK-92 cell migration depended on integrin binding site density and matrix metalloproteinase degradable sites in the hydrogel.
  • NK-92 cell migration and function varied with cancer cell type and culture duration, with reduced migration into metastatic H1299 models.
  • H1299 models exhibited increased immunosuppressive factors (TGF-β) and stress ligands, hindering NK-92 cell infiltration.
  • Inhibition of TGF-β receptor I in NK-92 cells significantly restored and enhanced their infiltration in both A549 and H1299 models.
  • NK-92 cell-mediated cytotoxicity was lower in 3D models compared to 2D cultures, indicating the hydrogel mimics in vivo biophysical barriers.

Conclusions:

  • The developed 3D hydrogel system effectively recapitulates key features of the solid tumor microenvironment, enabling the study of NK cell infiltration.
  • Tumor cell-derived factors, particularly TGF-β, play a critical role in suppressing NK cell function and migration.
  • Targeting TGF-β signaling presents a promising strategy to enhance NK cell-based immunotherapies for solid tumors.