Raman and Fluorescence Profiles Modifications of Muscular and Adipose Tissues Exposed to Low Energy X-ray Beams

Area of Science:

• Biophysics
• Spectroscopy
• Radiation Biology

Background:

• Low-energy radiation can induce biological changes.
• Autofluorescence and Raman spectroscopy are sensitive molecular analysis techniques.
• Understanding radiation effects on tissues is crucial for safety and medical applications.

Purpose of the Study:

• To investigate the effects of low-energy X-ray radiation on adipose and muscular tissues.
• To evaluate the utility of autofluorescence and Raman spectroscopy in detecting these radiation-induced changes.
• To assess biomolecular alterations at low radiation doses and energies.

Main Methods:

• Exposure of adipose and muscular tissue samples to X-ray beams at 25 and 35 kV.
• Application of radiation doses at 0.11, 1.1, and 2.1 Gy.
• Analysis using autofluorescence and Raman spectroscopy to detect molecular changes.

Main Results:

• Observed changes in Raman spectral bands associated with collagen, proteins, and lipids.
• Detected variations in autofluorescence emissions from collagen and nicotinamide adenine dinucleotide (NADH).
• Spectroscopic shifts indicated structural modifications and altered redox equilibrium of fluorophores.

Conclusions:

• Raman and fluorescence spectroscopy are effective tools for assessing low-energy radiation effects on biomolecules.
• These techniques can detect subtle changes in tissue composition and structure.
• The study demonstrates the sensitivity of spectroscopic methods for radiation biodosimetry.