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Improving the Anti-inflammatory Response via Gold Nanoparticle Vectorization of CO-Releasing Molecules

Alexandra R Fernandes1, Inês Mendonça-Martins1,2, Marino F A Santos2

  • 1UCIBIO, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal.

ACS Biomaterials Science & Engineering
|January 19, 2021
PubMed

Keywords:
CO-releasing moleculesCORM-3anti-inflammatory effectsbovine serum albumingold nanoparticlestissue regeneration

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Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.