Down-regulation of MALAT1 aggravates polycystic ovary syndrome by regulating MiR-302d-3p-mediated leukemia inhibitory factor activity
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Updated: Nov 20, 2025
Author Spotlight: Investigating the Mechanisms and Inducing Models of Polycystic Ovary Syndrome
Published on: July 5, 2024
Yafei Chen1, Yajie Chen2, Xiaoli Cui1
1Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450000, PR China.
Aims:
Accumulating evidence have shown the important roles of long noncoding RNA (lncRNA) in controlling different diseases. In the present study, we tried to explore the role which lncRNA MALAT1 plays in polycystic ovary syndrome (PCOS) with the involvement of microRNA-302d-3p (miR-302d-3p) and leukemia inhibitory factor (LIF).
Methods:
A PCOS rat model was established and characterized, followed by treatment with si-MALAT1, oe-MALAT1, miR-302d-3p mimic, or miR-302d-3p inhibitor constructs. Serum hormonal levels were detected to evaluate endocrine conditions. The effect of MALAT1 and miR-302d-3p on activities of ovarian granulosa cells was assessed, as well as the involvement of LIF.
Results:
MALAT1 expression was shown to be downregulated in ovarian tissue of PCOS rats. Overexpression of MALAT1 in vitro promoted proliferation and inhibited apoptosis of ovarian granulosa cells. Overexpression of MALAT1 in vivo reduced the ovarian tissue injury and endocrine disorders accompanied with decreased level of FSH and elevated serum levels of E2, T, and LH in the PCOS rat. Overexpression of MALAT1 also promoted the expression of LIF, which could be reversed by overexpression of miR-302d-3p, indicating that MALAT1 up-regulated the expression of LIF via miR-302d-3p. Furthermore, overexpression of MALAT1 reduced endocrine disorders and ovarian tissue damage via the miR-302d-3p/LIF axis.
Conclusion:
Our study highlighted that MALAT1 plays a protective role in reducing ovarian tissue damage and endocrine disorder in PCOS by regulating the miR-302d-3p/LIF axis.
Insights
Long noncoding RNA MALAT1 protects against polycystic ovary syndrome (PCOS) by regulating the microRNA-302d-3p/leukemia inhibitory factor axis. This study reveals MALAT1
Area of Science:
- Endocrinology
- Molecular Biology
- Genetics
Background:
- Polycystic ovary syndrome (PCOS) is a complex endocrine disorder.
- Long noncoding RNAs (lncRNAs) are increasingly recognized for their roles in disease pathogenesis.
- The specific role of lncRNA MALAT1 in PCOS requires further elucidation.
Purpose of the Study:
- To investigate the function of lncRNA MALAT1 in polycystic ovary syndrome (PCOS).
- To explore the involvement of microRNA-302d-3p (miR-302d-3p) and leukemia inhibitory factor (LIF) in the action of MALAT1 in PCOS.
Main Methods:
- Establishment and characterization of a PCOS rat model.
- Manipulation of MALAT1 and miR-302d-3p expression using si-MALAT1, oe-MALAT1, miR-302d-3p mimic, and inhibitor constructs.
- Assessment of serum hormone levels, ovarian granulosa cell activity, and LIF expression.
Main Results:
- MALAT1 expression was downregulated in PCOS rat ovarian tissue.
- Overexpression of MALAT1 promoted granulosa cell proliferation, inhibited apoptosis, and reduced ovarian injury and endocrine disorders in vivo.
- MALAT1 upregulated LIF expression via miR-302d-3p, suggesting a protective role through the miR-302d-3p/LIF axis.
Conclusions:
- lncRNA MALAT1 exerts a protective effect in PCOS.
- MALAT1 mitigates ovarian tissue damage and endocrine dysfunction by modulating the miR-302d-3p/LIF pathway.
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