Contribution of phenanthrene in different binding sites to its biodegradation in biochar-amended soils

Biochars can strongly sorb hydrophobic organic contaminants in soils. However, contribution of contaminants in different binding sites to their biodegradation in biochar-amended soils is not clear. In this work, wheat straw biochars were prepared at pyrolysis temperatures of 400 °C (BC400) and 700 °C (BC700). During a 42-day experiment, degradation rate constant of phenanthrene in soils was in the order of treatment without biochar (1.64 × 10^-2 d^-1) > treatment with BC700 (0.96 × 10^-2 d^-1) > treatment with BC400 (0.30 × 10^-2 d^-1). At the beginning, amendment of BC400 and BC700 reduced the rapidly desorbing fraction of phenanthrene in soils by 44.8% and 92.5%, respectively. At the end, both phenanthrene and microbial biomass highly concentrated on the biochar separated from soils. The results of a coupled model of desorption and biodegradation revealed that only phenanthrene in rapidly desorbing sites was degraded in BC400-amended soils, whereas degradation of phenanthrene in both rapidly and slowly desorbing sites occurred in BC700-amended soils, contributing 24.4% and 75.6% of the degradation, respectively. High fraction (>95%) of biodegradable phenanthrene in slowly desorbing sites was the key reason for higher biodegradation rate of phenanthrene in soils with BC700 than in soils with BC400.