Modulation of Intestinal Phosphate Transport in Young Goats Fed a Low Phosphorus Diet

The intestinal absorption of phosphate (P_i) takes place transcellularly through the active NaPi-cotransporters type IIb (NaPiIIb) and III (PiT1 and PiT2) and paracellularly by diffusion through tight junction (TJ) proteins. The localisation along the intestines and the regulation of P_i absorption differ between species and are not fully understood. It is known that 1,25-dihydroxy-vitamin D₃ (1,25-(OH)₂D₃) and phosphorus (P) depletion modulate intestinal P_i absorption in vertebrates in different ways. In addition to the apical uptake into the enterocytes, there are uncertainties regarding the basolateral excretion of P_i. Functional ex vivo experiments in Ussing chambers and molecular studies of small intestinal epithelia were carried out on P-deficient goats in order to elucidate the transepithelial P_i route in the intestine as well as the underlying mechanisms of its regulation and the proteins, which may be involved. The dietary P reduction had no effect on the duodenal and ileal P_i transport rate in growing goats. The ileal PiT1 and PiT2 mRNA expressions increased significantly, while the ileal PiT1 protein expression, the mid jejunal claudin-2 mRNA expression and the serum 1,25-(OH)₂D₃ levels were significantly reduced. These results advance the state of knowledge concerning the complex mechanisms of the P_i homeostasis in vertebrates.