Related Experiment Video
Updated: Nov 20, 2025
Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology
Published on: March 23, 2011
Effects of Late-Life Caloric Restriction on Age-Related Alterations in the Rat Cortex and Hippocampus
Claudia Tonini1, Marco Segatto2, Francesca Martino1
1Department of Science, University Roma Tre, Viale Marconi 446, 00146 Rome, Italy.
Background:
A major problem of aging is the disruption of metabolic homeostasis. This is particularly relevant in the brain where it provokes neurodegeneration. Caloric restriction is a physiologic intervention known to delay the deleterious consequences of aging in several species ranging from yeast to mammals. To date, most studies on experimental models have started this dietary intervention from weaning, which is very difficult to be translated to human beings. Here, we study the effects of a more realistic dietary regimen in rats, starting at an advanced age and lasting for six months.
Methods:
we analyzed in the cortex and hippocampus, the proteins involved in the energetic balance of the cells, cholesterol metabolism, oxidative stress response, inflammation, synaptic impairment, and brain trophism.
Results:
our results suggest that caloric restriction in late life can revert only some age-related changes studied here.
