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A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain
Published on: July 25, 2022
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Modular vaccine platform based on the norovirus-like particle
Vili Lampinen1, Suvi Heinimäki2, Olli H Laitinen1
1Faculty of Medicine and Health Technology, Tampere University, 33014, Tampere, Finland.
Journal of Nanobiotechnology
|January 20, 2021
Summary
This study presents a novel modular norovirus-like particle (VLP) vaccine platform. This platform rapidly and safely generates high antibody titers against decorated influenza antigens in mice.
Area of Science:
- Vaccinology
- Immunology
- Structural Biology
Background:
- Virus-like particle (VLP) vaccines offer a safe and effective alternative to traditional vaccines.
- VLPs can be engineered to display pathogen antigens, enhancing immunogenicity.
- Separate production of VLP platforms and antigens allows for rapid vaccine development.
Purpose of the Study:
- To develop a modular norovirus-like particle (VLP) vaccine platform.
- To decorate noro-VLPs with conserved influenza antigens.
- To assess the immunogenicity of these vaccine candidates in BALB/c mice.
Main Methods:
- SpyTagged noro-VLPs were expressed in insect cells and purified using scalable methods.
- Conserved influenza antigens were produced as SpyCatcher fusions in E. coli.
- Antigens were covalently conjugated to the noro-VLP surface.
Main Results:
- SpyTagged noro-VLP expression and purification were highly efficient.
- The engineered noro-VLPs demonstrated stability comparable to native noro-VLPs.
- The noro-VLP platform induced high antibody titers against the presented influenza antigens, indicating a strong adjuvant effect.
Conclusions:
- The developed noro-VLP platform provides a rapid, convenient, and safe method for vaccination.
- This modular platform facilitates the presentation of diverse soluble protein antigens for immune system engagement.
- It is suitable for vaccine development and antibody production purposes.
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