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Related Concept Videos

Glucose Homeostasis: Pancreatic Islets and Insulin Secretion01:27

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Related Experiment Video

Updated: Nov 20, 2025

Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro
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Dynamic Uni- and Multicellular Patterns Encode Biphasic Activity in Pancreatic Islets.

Manon Jaffredo1, Eléonore Bertin1, Antoine Pirog2

  • 1University of Bordeaux, CNRS, Institute of Chemistry and Biology of Membranes and Nano-objects, UMR 5248, Pessac, France.

Diabetes
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PubMed
Summary
This summary is machine-generated.

Islet beta cells exhibit biphasic secretion for glucose control. This study reveals distinct multicellular electrical organization for first and second phases, crucial for understanding type 2 diabetes.

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Area of Science:

  • Endocrinology
  • Cellular Biology
  • Neuroscience

Background:

  • Biphasic secretion by islet beta cells is vital for glucose homeostasis.
  • Dysregulation of this activity is linked to type 2 diabetes.
  • The precise multicellular organization driving biphasic secretion remains incompletely understood.

Purpose of the Study:

  • To investigate the uni- and multicellular functional organization of islet beta-cell activation during both first and second phases of secretion.
  • To model beta-cell activation at physiological and pathophysiological conditions.

Main Methods:

  • Utilized high-resolution, noninvasive multielectrode array recordings for long-term, simultaneous analysis of single-cell and coupling activity in mouse and human islets.
  • Examined beta-cell activity under physiological conditions and in models of aging and glucotoxicity.

Main Results:

  • Identified distinct organizational modes for the first and second phases of secretion.
  • The first phase is characterized by small, highly active beta-cell clusters.
  • The second phase involves electrical coupling generating larger functional clusters via multicellular slow potentials, promoting sustained activity.
  • Glucagon-like peptide 1 enhances coupling in the second phase, while aging and glucotoxicity disrupt coupling in both phases.

Conclusions:

  • Biphasic secretion is regulated at the micro-organ level by multicellular electrical signals and beta-cell synchronization.
  • A novel model of beta-cell activation highlights dynamic multicellular electrical organization.
  • Altered electrical organization in islets may underlie functional deficits observed in type 2 diabetes.