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Pannexin 3 regulates skin development via Epiprofin
Peipei Zhang1, Masaki Ishikawa2, Andrew Doyle3
1Molecular Biology Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, 20892, USA.
Scientific Reports
|January 20, 2021
Summary
Pannexin 3 (Panx3) is crucial for skin development and hair follicle regeneration. This study shows Panx3 regulates skin by modulating Epiprofin (Epfn) expression and activating key signaling pathways.
Area of Science:
- Dermatology
- Cell Biology
- Developmental Biology
Background:
- Pannexin 3 (Panx3) is essential for hard tissue formation.
- The function of Panx3 in skin development is not well understood.
Purpose of the Study:
- To investigate the role of Panx3 in skin development and hair follicle regeneration.
- To elucidate the molecular mechanisms by which Panx3 influences skin development.
Main Methods:
- Utilized Panx3 knockout (Panx3-/-) mice and shRNA to suppress Panx3 expression.
- Investigated Epiprofin (Epfn) expression in epidermal cells.
- Performed cell culture experiments with HaCaT cells overexpressing Panx3.
- Examined the Akt/NFAT signaling pathway in keratinocytes.
Main Results:
- Panx3 knockout mice exhibited impaired skin development and delayed hair follicle regeneration.
- Reduced Panx3 expression led to decreased Epfn expression in the epidermis.
- Panx3 overexpression enhanced HaCaT cell differentiation and Epfn expression.
- Panx3 activation of the Akt/NFAT pathway promotes keratinocyte differentiation.
Conclusions:
- Panx3 plays a critical role in skin development and hair follicle regeneration.
- Panx3 regulates skin development primarily by modulating Epiprofin (Epfn) expression.
- Panx3 influences keratinocyte differentiation through the Akt/NFAT signaling pathway.
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