Related Experiment Video
Updated: Nov 20, 2025
07:02
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
Published on: August 23, 2019
7.6K
CRNDE Contributes Cervical Cancer Progression by Regulating miR-4262/ZEB1 Axis
Lu Ren1, Shaoqin Yang1, Qinxue Cao1
1Department of Obstetrics and Gynecology, Huaihe Hospital of Henan University, Kaifeng, 475001 Henan, People's Republic of China.
Oncotargets and Therapy
|January 20, 2021
Summary
Long non-coding RNA CRNDE promotes cervical cancer by sponging miR-4262 and upregulating ZEB1, activating the wnt/β-catenin pathway. This study reveals CRNDE as a potential therapeutic target for cervical cancer.
Area of Science:
- Gynecologic Oncology
- Molecular Biology
- Cancer Research
Background:
- Cervical cancer is a significant cause of mortality in women.
- Long non-coding RNA colorectal neoplasia differentially expressed (LncRNA CRNDE) is an oncogene in various cancers.
- The role of LncRNA CRNDE in cervical cancer pathogenesis is not well understood.
Purpose of the Study:
- To investigate the role of LncRNA CRNDE in cervical cancer.
- To elucidate the molecular mechanism of CRNDE in cervical cancer progression.
- To explore the potential of targeting the CRNDE/miR-4262/ZEB1 axis for cervical cancer therapy.
Main Methods:
- Quantitative real-time polymerase chain reaction (qRT-PCR) to measure gene expression.
- Cell viability, apoptosis, migration, and invasion assays were performed.
- Dual-luciferase reporter system and Western blot were used to confirm molecular interactions and protein expression.
Main Results:
- CRNDE and ZEB1 were upregulated, while miR-4262 was downregulated in cervical cancer.
- CRNDE acts as a sponge for miR-4262, and ZEB1 is a target of miR-4262.
- CRNDE promotes cervical cancer progression by regulating the miR-4262/ZEB1 axis and activating the wnt/β-catenin pathway.
Conclusions:
- CRNDE facilitates cervical cancer progression via the wnt/β-catenin pathway by modulating the miR-4262/ZEB1 axis.
- The CRNDE/miR-4262/ZEB1 pathway represents a promising therapeutic target for cervical cancer treatment.
Related Concept Videos
01:06Induced Pluripotent Stem Cells
4.9K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
Somatic...
4.9K
01:51Mechanisms of Retrovirus-induced Cancers
5.9K
Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
5.9K
01:22MicroRNAs
3.4K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.4K
01:22MicroRNAs
23.2K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.2K
02:23Abnormal Proliferation
4.9K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.9K
02:07Tumor Progression
6.9K
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
6.9K