In vitro bursting strength studies of laser-welded tissue and comparison with conventional anastomosis

We compared the mechanical integrity of microvascular anastomoses created with a carbon dioxide (CO2) laser with conventional suture anastomoses. Seventy rat femoral artery segments (35 lased and 35 sutured) were harvested at 1, 24, and 72 hours, and 1, 3, 6, and 12 weeks postoperatively. These segments were subjected to increasingly higher in vitro intraluminal hydrostatic pressures (bursting pressure). Conventionally sutured anastomoses exhibited significantly increased ability (p less than 0.05) to withstand greater bursting pressures than the laser-welded tissue up to 3 weeks postoperatively. After the third postoperative week, the laser anastomoses demonstrated increased bursting pressures compared with the conventional anastomoses. At the end of the 12-week period both groups demonstrated an ability to withstand supraphysiologic pressures in excess of 2000 mm Hg.