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LF1 regulates the lateral organs polarity development in rice
Ting Zhang1, Jing You1, Yi Zhang1
1Key Laboratory of Application and Safety Control of Genetically Modified Crops, Rice Research Institute, Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China.
The New Phytologist
|January 20, 2021
Summary
The rice LF1 gene, a homeodomain-leucine zipper III (HD-ZIP III) factor, regulates lateral organ polarity. It forms negative feedback loops with OsZPR4 and OsHOX1, impacting auxin levels and development.
Area of Science:
- Plant Molecular Biology
- Developmental Biology
- Genetics
Background:
- Adaxial-abaxial tissue patterning is crucial for lateral organ morphology.
- Homeodomain-leucine zipper III (HD-ZIP III) genes specify adaxial identity, but their interactions and downstream targets are not fully understood.
Purpose of the Study:
- To investigate the molecular mechanisms underlying lateral organ polarity development in rice.
- To identify downstream targets and interaction partners of HD-ZIP III transcription factors.
Main Methods:
- Identification and characterization of a dominant rice mutant, lateral floret 1 (lf1).
- Analysis of gene expression, protein-protein interactions, and hormone content.
- Investigating the role of LF1 in regulating downstream genes like OsZPR4, OsHOX1, and OsYUCCA6.
Main Results:
- The lf1 mutant exhibits defects in lateral organ polarity.
- LF1 directly activates OsZPR4 (LITTLE ZIPPER family) and OsHOX1 (HD-ZIP II family) expression.
- OsZPR4 and OsHOX1 interact with LF1 to form heterodimers, repressing LF1's transcriptional activity.
- LF1 regulates indole-3-acetic acid (IAA) content by controlling OsYUCCA6 expression.
Conclusions:
- LF1 establishes negative feedback loops with OsZPR4 and OsHOX1, influencing IAA levels and lateral organ polarity.
- Reveals cross-talk between HD-ZIP III, LITTLE ZIPPER, and HD-ZIP II proteins in plant development.
- Provides new insights into the molecular mechanisms governing lateral organ polarity.
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