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Collateral sensitivity associated with antibiotic resistance plasmids.

Cristina Herencias1, Jerónimo Rodríguez-Beltrán1,2, Ricardo León-Sampedro1,2

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Summary

Collateral sensitivity (CS) can combat antibiotic resistance (ABR). This study shows CS can target bacteria carrying resistance plasmids, offering new strategies against plasmid-mediated ABR.

Keywords:
E. coliantibioticscollateral sensitivityevolutionary biologyinfectious diseasemicrobiologyplasmid

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Antibiotic resistance (ABR) is a growing global health threat.
  • Collateral sensitivity (CS) offers a strategy where resistance to one antibiotic increases susceptibility to another.
  • Existing CS research primarily targets chromosomal mutations, overlooking plasmid-mediated ABR.

Purpose of the Study:

  • To investigate collateral sensitivity (CS) in bacteria acquiring complete antibiotic resistance (ABR) plasmids.
  • To analyze CS associated with the clinically significant carbapenem-resistance plasmid pOXA-48.
  • To explore the application of CS in clinical isolates for selective killing of plasmid-carrying bacteria.

Main Methods:

  • Analysis of CS in bacteria harboring complete antibiotic resistance plasmids.
  • Characterization of CS associated with the pOXA-48 carbapenem-resistance plasmid.
  • Evaluation of CS conservation in clinical *E. coli* isolates.

Main Results:

  • First analysis of CS linked to the acquisition of complete antibiotic resistance plasmids.
  • Demonstrated CS associated with the pOXA-48 plasmid.
  • Confirmed conservation of CS in clinical *E. coli* and its utility for selective killing.

Conclusions:

  • CS is a viable strategy against plasmid-mediated antibiotic resistance.
  • CS can be leveraged to selectively eliminate bacteria carrying resistance plasmids.
  • Findings provide a foundation for CS-informed treatments against plasmid-mediated ABR.