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Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

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Related Experiment Video

Updated: Nov 20, 2025

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

A Rapid Method for Stably Transforming Rice Seeds.

Sudheer Kumar¹, Neeti Sanan-Mishra²

¹Plant RNAi Biology Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India.

Methods in Molecular Biology (Clifton, N.J.)

|January 20, 2021

Summary

This study presents a new, efficient method for rice transformation using Agrobacterium, bypassing traditional tissue culture. The developed technique enables direct transformation of rice seeds, yielding stable transgenes in the first generation.

Keywords:

Agrobacterium tumefaciens Artificial microRNA In planta Rice seeds Transformation

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Area of Science:

Agricultural Biotechnology
Plant Molecular Biology
Genetics and Genomics

Background:

Agrobacterium-mediated plant transformation is crucial for research but often relies on time-consuming tissue culture methods.
Existing rice transformation protocols are limited by labor requirements and tissue regeneration capacity.

Purpose of the Study:

To develop a simple, robust, and tissue culture-independent method for rice seed transformation.
To demonstrate the efficacy of the new method using a model construct (pCAMBIA-amiR820).

Main Methods:

Transformation of rice seeds directly using Agrobacterium.
Selection of transformed plants on antibiotic media.
Molecular analysis for transgene integration and expression.

Main Results:

A tissue culture-independent transformation method for rice seeds was successfully established.
Transgenic seedlings showed a mix of stable transformants and chimeras.
Stable transgene integration was confirmed in the first generation seeds.

Conclusions:

The developed method offers a simplified and efficient alternative for rice transformation.
This approach overcomes limitations associated with traditional tissue culture-dependent protocols.
The method facilitates basic and translational research in rice by enabling straightforward genetic modification.