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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Related Experiment Video

Updated: Nov 20, 2025

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
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Single Transcript Unit CRISPR 2.0 Systems for Genome Editing in Rice.

Xu Tang1, Yiping Qi2,3, Yong Zhang4

  • 1Department of Biotechnology, Center for Informational Biology, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.

Methods in Molecular Biology (Clifton, N.J.)
|January 20, 2021
PubMed
Summary
This summary is machine-generated.

We present a new CRISPR 2.0 toolbox for efficient single and multiplex genome editing in rice. This system utilizes single transcript unit (STU) CRISPR-Cas9 and CRISPR-Cas12a technologies for advanced plant gene modification.

Keywords:
CRISPR-CasCas12aCas9Csy4Genome editingGolden GateSTU CRISPR 2.0tRNA

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Area of Science:

  • Plant biotechnology
  • Molecular biology
  • Genome editing

Background:

  • CRISPR-Cas9 and CRISPR-Cas12a are powerful RNA-guided DNA endonucleases used for genome editing in plants.
  • These systems are derived from prokaryotic adaptive immune systems.
  • Efficient genome editing is crucial for crop improvement and research.

Purpose of the Study:

  • To develop and detail a protocol for using an improved CRISPR 2.0 toolbox in rice.
  • To demonstrate the capability of single transcript unit (STU) CRISPR systems for plant genome engineering.
  • To enable both single and multiplex genome editing applications in rice.

Main Methods:

  • Development of a CRISPR 2.0 toolbox containing two STU-Cas9 and one STU-Cas12a systems.
  • Application of the STU CRISPR 2.0 systems for genome editing in rice.
  • Detailed protocol description for single and multiplex editing strategies.

Main Results:

  • Successful implementation of the STU CRISPR 2.0 toolbox in rice.
  • Demonstration of efficient single and multiplex genome editing capabilities.
  • Establishment of a reliable protocol for researchers to utilize these advanced tools.

Conclusions:

  • The STU CRISPR 2.0 toolbox provides an effective platform for advanced genome editing in rice.
  • This system facilitates precise genetic modifications for research and crop development.
  • The detailed protocol empowers the wider adoption of CRISPR technology in plant science.