Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Methylations in human hemoglobin.

M Törnqvist1, S Osterman-Golkar, A Kautiainen

  • 1Department of Radiobiology, University of Stockholm, Sweden.

Mutation Research
|March 1, 1988
PubMed
Summary

N-Methylvaline (MeVal) in hemoglobin (Hb) was measured in smokers and non-smokers. MeVal levels were consistent across individuals and species, unaffected by smoking, unlike other alkylations.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhanced susceptibility of obese mice to glycidamide-induced sperm chromatin damage without increased oxidative stress.

Andrology·2016
Same author

Mobile health in emerging countries: a survey of research initiatives in Brazil.

International journal of medical informatics·2013
Same author

Benign prostatic hyperplasia and prostate cancer: an overview for primary care physicians.

International journal of clinical practice·2010
Same author

Inhibitory effect of tetranitromethane on the diphtheria toxin-antitoxin precipitin reaction.

Nature·2010
Same author

Influence of temperature on the nucleolus and its coacervate nature.

Hereditas·2010
Same author

The shape of the spindle at metaphase is conditioned by the shape of its molecules.

Hereditas·2010

Area of Science:

  • Biochemistry
  • Toxicology
  • Biomarker Research

Background:

  • Hemoglobin (Hb) alkylations serve as biomarkers for genotoxic agent exposure.
  • Understanding background levels of these modifications is crucial for accurate exposure assessment.

Purpose of the Study:

  • To quantify N-Methylvaline (MeVal) and N tau-methylhistidine (MeHis) levels in human and animal hemoglobin.
  • To investigate the influence of smoking on MeVal levels.
  • To explore potential endogenous sources of MeVal.

Main Methods:

  • Measurement of MeVal and MeHis in hemoglobin from male smokers and non-smokers.
  • Quantification of MeVal in hemoglobin from various animal species (rats, hamsters, mice, chickens).
  • Comparison of MeVal levels with other hemoglobin adducts like N-hydroxyethylvaline (HOEtVal) and S-methylcysteine (MeCys).

Related Experiment Videos

Main Results:

  • MeVal was detected at approximately 0.5 nmole/g Hb with minimal inter-individual and inter-species variation.
  • Smoking did not significantly alter MeVal levels in human hemoglobin.
  • Levels of S-methylcysteine (MeCys) and MeHis were substantially higher than MeVal, potentially due to protein synthesis misincorporation or artifacts.
  • One smoker exhibited outlier levels of both MeVal and HOEtVal, suggesting a possible defective detoxification system.

Conclusions:

  • MeVal is a stable hemoglobin alkylation with low background levels, not significantly influenced by smoking.
  • MeVal is a poor indicator for smoking-induced exposure compared to other adducts.
  • Further investigation into endogenous sources like S-adenosylmethionine and formaldehyde for MeVal is warranted.
  • The outlier case highlights the potential of combined adduct analysis in identifying individuals with impaired detoxification pathways.