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miRNA-128 modulates bone neoplasms cells proliferation and migration through the WNT/β-catenin and EMT signal

Yang Li1, Xiaotao Long1, Ji Wang1

  • 1Department of Orthopedics, Chongqing General Hospital, University of the Chinese Academy of Sciences, No. 312 Zhongshanyi Road, Yuzhong District, Chongqing, 400013, China.

Journal of Orthopaedic Surgery and Research
|January 21, 2021
PubMed
Abstract

Insights

MicroRNA-128 (miR-128) suppresses bone neoplasm growth and metastasis by inhibiting Wnt/β-catenin and epithelial-mesenchymal transition (EMT) pathways. This finding offers a potential gene therapy target for bone neoplasms.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Therapy

Background:

  • Bone neoplasms are aggressive with high recurrence and metastasis rates.
  • MicroRNAs (miRNAs) show potential in inhibiting bone neoplasm progression.
  • The specific role of microRNA-128 (miR-128) in bone neoplasm invasiveness requires further elucidation.

Purpose of the Study:

  • To investigate the regulatory mechanisms of miR-128 in bone neoplasm cells.
  • To understand how miR-128 influences cancer cell proliferation, migration, and invasion.

Main Methods:

  • Western blotting and qRT-PCR to analyze protein and RNA levels.
  • Cell proliferation, migration, and invasion assays (e.g., CCK-8, TUNEL).
  • Luciferase assays, immunofluorescence, and immunohistochemistry to assess pathway activation.

Main Results:

  • miR-128 transfection suppressed bone neoplasm cell proliferation, migration, and invasion.
  • miR-128 knockdown activated Wnt/β-catenin and epithelial-mesenchymal transition (EMT) pathways.
  • Wnt/EMT pathway activation counteracted miR-128's inhibitory effects.
  • Exogenous miR-128 significantly inhibited tumor growth in xenograft models.

Conclusions:

  • miR-128 down-regulates Wnt/β-catenin and EMT signaling in bone neoplasms.
  • miR-128 exhibits tumor-suppressive functions in bone neoplasms.
  • Targeting miR-128 presents a potential strategy for bone neoplasm gene therapy.
Keywords:
Bone neoplasmsEMTMigrationProliferationWnt/β-cateninmiRNA-128

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