Jove
Visualize
Contact Us

Related Experiment Videos

Induction of yeast killer factor mutations.

M Vodkin

    Journal of Bacteriology
    |October 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Mutagenesis of killer yeast strains revealed that double-stranded ribonucleic acid (dsRNA) is essential for killer activity. Some mutants lost all dsRNA, while others showed altered dsRNA profiles, indicating its role in killer phenotype.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Isolated Coxiella burnetii synthesizes DNA during acid activation in the absence of host cells.

    Journal of general microbiology·1990
    Same author

    Sequence and analysis of the DNA encoding protective antigen of Bacillus anthracis.

    Gene·1988
    Same author

    Site-specific base substitution and deletion mutations that enhance or suppress transcription of the SV40 major late RNA.

    Cell·1982
    Same author

    Homology between double-stranded RNA and nuclear DNA of yeast.

    Journal of virology·1977
    Same author

    Yeast killer mutants with altered double-stranded ribonucleic acid.

    Journal of bacteriology·1974
    Same author

    Divergence of Ribosomal RNA Sequences within Angiospermae.

    Genetics·1971
    JoVE
    x logofacebook logolinkedin logoyoutube logo
    ABOUT JoVE
    OverviewLeadershipBlogJoVE Help Center
    AUTHORS
    Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
    LIBRARIANS
    TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
    RESEARCH
    JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
    EDUCATION
    JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
    Terms & Conditions of Use
    Privacy Policy
    Policies

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Yeast Genetics

    Background:

    • Killer strains of Saccharomyces cerevisiae produce toxins.
    • These killer phenotypes are often associated with the presence of double-stranded ribonucleic acid (dsRNA) molecules.
    • Understanding the genetic basis of killer activity is crucial for yeast research.

    Purpose of the Study:

    • To investigate the role of double-stranded ribonucleic acid (dsRNA) in the killer phenotype of Saccharomyces cerevisiae.
    • To identify nonkiller variants and analyze their genetic makeup.

    Main Methods:

    • Mutagenesis of two related killer yeast strains.
    • Screening of mutagenized populations for nonkiller variants.
    • Analysis of double-stranded ribonucleic acid (dsRNA) content in selected mutants.

    Related Experiment Videos

    Main Results:

    • Approximately 20% of mutants from one strain lost all detectable double-stranded ribonucleic acid (dsRNA).
    • Around 70% of mutants from the other strain exhibited a different dsRNA profile, lacking a specific species and possessing a lower molecular weight one.
    • These findings correlate dsRNA presence and integrity with killer activity.

    Conclusions:

    • Double-stranded ribonucleic acid (dsRNA) is essential for maintaining the killer phenotype in Saccharomyces cerevisiae.
    • Specific dsRNA species are directly linked to the killer factor.
    • Mutations affecting dsRNA can lead to the loss or alteration of killer activity.