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Updated: Nov 20, 2025

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
The complete mitochondrial genome of the Songpu mirror carp, Cyprinus carpio
Quan Huang1,2, Zhen Wang2
1College of fisheries, Huazhong Agricultural University, China.
Abstract:
In this study, the complete mitochondrial genome sequences of Cyprinus carpio (strain Songpu) were reported for the first time. The genome is found to be 16 582 bp in length accurately using the next-generation sequencing technology and bioinformatics tools, and it has a base composition of A (31.9%), G (15.7%), C (27.5%) and T (24.9%), indicating that the percentage of A + T (56.8%) was higher than G + C (43.2%). The whole genome contains a typically conserved structure, which includes 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes and one control region (D-loop). To obtain the phylogenetic relationship between Songpu mirror carp and other species, 11 mitochondrial genomes were used for phylogenetic analysis. The result shows the Songpu mirror carp is closely related to Cyprinu carpio carpio that is also a member of the genus Cyprinu.
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