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Updated: Nov 20, 2025

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
The analysis for mitochondrial genome sequencing of Triplophysa lewanggensis
Huaiqing Deng1, Huamei Wen1, Ning Xiao2
1School of Life Sciences, Guizhou Normal University, Guiyang, Guizhou, China.
Abstract:
Triplophysa lewanggensis revealed that the complete length of its mitochondrial genome was 16,568 bp, composed of A (31.7%), T (27.1%), G (15.9%), C (25.4%), and A + T (58.8%). Its genetic constitution and arrangement were consistent with other Triplophysa, including 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 1 control area (D-loop). All genes were encoded by the H-strand, except for one protein-coding gene (ND6) and eight tRNA genes (tRNAGln, tRNAAla, tRNACys, tRNAAsn, tRNATyr, tRNASer(UCN), tRNAGlu, tRNAPro) are encoded by the L chain, and the remaining genes are encoded by the H chain. The phylogenetic tree was divided into two main clades: one just including Triplophysa and the other including Oreonectes, Homatula, Schistura, Nemacheilus, Traccatichthys. T. lewanggensis is distributed in Southwest Guizhou Province attached to Yunnan-Guizhou Plateau, T. xiangxiensis is distributed in Hunan Province, they are near relatives, but their kinship with Triplophysa distributed in Tibetan Plateau is farther.
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