DNA barcoding of mayflies (Insecta: Ephemeroptera) from South India

In this study, DNA barcodes were generated for 40 species belonging to 32 genera under 10 families of Ephemeroptera from South India. Nucleotide sequence divergences were calculated using the Kimura two-parameter distance model and a neighbour-joining analysis was performed to provide a graphic display of the patterns of divergence among the species. This study demonstrates that COI barcoding is effective in discriminating among the mayfly species of South India, and provides a reference library for their future molecular identification.