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Updated: Nov 20, 2025

Novel Sequence Discovery by Subtractive Genomics
Published on: January 25, 2019
Complete sequencing of the chloroplast genomes of two Medicago species
Yan Weihong1, Shi Wengui1, Liu Lei1
1Ministry of Agriculture, Institute of Grassland Research of CAAS/Key Laboratory of Grassland Resources and Utilization, Inner Mongolia, Hohhot, China.
Abstract:
In this study, we aimed to determine the complete nucleotide sequences of the chloroplast genomes of Medicago sativa L. and Medicago falcata L. and perform their phylogenomic analysis. The chloroplast genomes of M. Sativa and M. falcata were found to be 125,095 and 125,810 bp in length, respectively. Additionally, the AT contents of M. Sativa and M. falcata are 66.1% and 66.2%, respectively. Each chloroplast genome contained the same 109 unique genes, including 29 transfer RNA genes, 4 ribosomal RNA genes, and 76 protein-coding genes. Phylogenomic analysis based on complete chloroplast genomes from the Medicago showed that the geographical environment has no significant effect on their genomic composition.
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