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Updated: Nov 20, 2025

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
Characterization of the complete mitochondrial genome of Diodon hystrix with phylogenetic consideration
Hai Huang1,2, Hong Guang3, Jun Ma1,2
1Hainan Key Laboratory for Conservation and Utilization of Tropical Marine Fishery Resources, Sanya, Hainan, China.
Abstract:
In this study, the complete mitochondrial genome of Diodon hystrix was sequenced and analyzed. The mitochondrial genome is 16,512 bp long and consists of 13 protein-coding genes, two rRNA genes, 22 tRNA genes and a control region. The gene order and composition of D. hystrix mitochondrial genome was similar to that of most other vertebrates. The nucleotide composition of the light strand in descending order is 28.69% of A, 31.64% of C, 23.66% of T and 16% of G. The NADH dehydrogenase subunit 6 (ND6) and 8 tRNA genes were localized in the light strand, and all other mitochondrial genes were encoded on the heavy strand. The phylogenetic analysis by maximum-likelihood (ML) method revealed that D. hystrix has the closer relationship to the Diodon holocanthus.
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