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Updated: Nov 20, 2025

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications
Published on: November 5, 2020
The complete mitochondrial genome sequence and gene organization of Ambassis gymnocephalus (Perciformes, Ambassidae)
Yao Dong1,2, Chunyan Ma1, Hongyu Ma1,3
1Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China.
Abstract:
In this study, we firstly obtained the complete mitochondrial genome DNA sequence of the Ambassis gymnocephalus. Its length is 17,388 bp, containing 13 protein-coding genes, 2 rRNAs, 22 tRNAs and a large noncoding region. The overall length of protein coding genes is 11,448 bp, including A (26.75%), G (15.20%), T (26.44%) and C (31.61%). The A + T content of control region (1555 bp) is 60.45%. The protein-coding gene COI is started by GTG and ended by AGA. From the phylogenetic tree, we find A. gymnocephalus and Oreochromis niloticus has the closest relationship among 21 related species. This work will provide some valuable information for further studies on A. gymnocephalus and the family Ambassidae.
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