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Updated: Nov 20, 2025

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
Published on: July 28, 2017
The complete chloroplast genome of Fraxinus chiisanensis (Oleaceae)
Sang-Chul Kim1, Jei-Wan Lee1, Seung-Hoon Baek1
1Division of Forest Genetic Resources, National Institute of Forest Science, Suwon, Republic of Korea.
Abstract:
Fraxinus chiisanensis is endemic to Korea and restricted to local habitats with an extremely limited population size. The chloroplast genomic information can be used to formulate a comprehensive conservation strategy. In this study, the complete chloroplast (cp) genome sequence of F. chiisanensis was determined using next-generation sequencing. The entire cp genome was determined to be 155,571 bp in length. It contained large single-copy (LSC) and small single-copy (SSC) regions of 86,412 of 17,767 bp, respectively, which were separated by a pair of 25,696 bp inverted repeat (IR) regions. The genome contained 132 genes, including 87 protein-coding genes, 37 tRNA genes, and eight rRNA genes. Among the 132 genes, seven coding genes, seven tRNA genes, and four rRNA genes occur in the two IR regions. The phylogenetic position of F. chiisanensis in Oleaceae was closely clustered with Olea, Chionanthus retusus, and Hesperelaea palmeri as sister species.
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