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Updated: Nov 20, 2025

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
Complete mitochondrial genome of Taiwanese loach, Paramisgurnus dabryanus ssp. (Cobitinae)
Xia Liang1, Daoyu Zhu1, Xiaodong Li2
1Key Laboratory for Physiology Biochemistry and Application, School of Agriculture and Bioengineering, Heze University, Heze, Shandong, China.
Abstract:
Taiwanese loach was bred in Formosa and then widely cultivated in China. Because of overfishing and environmental pollution, the number of wild Taiwanese loach has been sharply decreased in these years. Also the taxonomic status of Taiwanese loach is still unclear. In this study, the complete mitochondrial genome of Taiwanese loach was obtained by PCR. The genome is 16,569 bp in length, including 2 ribosomal RNA genes, 13 proteins-coding genes, 22 transfer RNA genes, and a non-coding control region, the gene composition and order of which was similar to most reported from other vertebrates. Sequence analysis showed that the overall base composition is 29.5% for A, 27.5% for T, 26.4% for C, and 16.6% for G. The sequence is a slight A + T bias of 57.0%. By analyzing phylogenetic analysis and BLAST, the similarity to Paramisgurnus was >99%, we dare to speculate that the Taiwanese loach cultivated in China was a subspecies of P. dabryanus. Mitogenome information from this study could be a useful basis for conservation and phylogenetics of Taiwanese loach, P. dabryanus ssp.
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