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Updated: Nov 20, 2025

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Published on: March 8, 2018
Complete chloroplast genome of an endangered tree species, Toona ciliata (Sapindales: Meliaceae)
Gui-Liang Xin1, Jia-Qian Liu1, Jia Liu1
1Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, School of Life Science, Northwest University, Xi'an, China.
Abstract:
The whole cp genome of T. ciliata was 159,502 bp in length, containing a pair of inverted repeat (26,961 bp for each), a large single copy (87,199 bp) and a small single copy (18,381 bp) regions. The cp genome encoded 138 genes, including 89 protein-coding genes, 40 tRNA genes, 8 rRNA genes and 1 pseudogene. The nucleotide composition was asymmetric (30.7% A, 19.3% C, 18.6% G and 31.4% T) with an overall GC content of 37.9%. The maximum likelihood phylogenetic analysis based on 21 complete cp genome sequences showed that T. ciliata closely related to Azadirachta indica.
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