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Updated: Nov 20, 2025

A Whole Mount In Situ Hybridization Method for the Gastropod Mollusc Lymnaea stagnalis
Published on: March 15, 2016
The complete mitochondrial genome of the apple snail Pomacea maculate (Gastropoda: Ampullariidae)
Huirong Yang1, Jia-En Zhang2,3,4, Zhixin Deng2
1College of Marine Sciences, South China Agricultural University, Guangzhou, China.
Abstract:
We present the complete mitochondrial genome of Pomacea maculate in this study. The mitochondrial genome is 15,512 bp in length, containing 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes. Overall nucleotide compositions of the light strand are 41.13% of A, 30.81% of T, 15.25% of C and 12.81% of G. Its gene arrangement and distribution are different from the typical vertebrates. The absence of D-loop is consistent with the Gastropoda, but at least one lengthy non-coding region is essential regulatory element for the initiation of transcription and replication. Phylogenetic tree is constructed by the maximum-likelihood method based on the complete mitochondrial genomes of 15 species of Caenogastropoda, using Helix aspersa as outgroup to assess their actual phylogenetic relationship and evolution. The result provides fundamental data for resolving phylogenetic and genetic problems related to effective management strategies.
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