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Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Published on: March 8, 2018
The complete chloroplast genome sequence of the medicinal plant Fagopyrum dibotrys (Polygonaceae)
1Institute of Botany of Shaanxi Province, Xi'an Botanical Garden of Shaanxi Province, Xi'an, Shaanxi Province, China.
Abstract:
Chloroplast (cp) genome sequences become a useful popular tool for population and phylogeny in recent reports. Here, the complete chloroplast genome of the Fagopyrum dibotrys has been reconstructed from the whole-genome Illumina sequencing data. The circular genome is 159,325 bp in size, and comprises a pair of inverted repeat (IR) regions of 67,788 bp each, a large single-copy (LSC) region of 84,593 bp, and a small single-copy (SSC) region of 6,944 bp. The total Guanine and Cytosine (GC) content is 38.0%, while the corresponding values of the LSC, SSC, and IR region are 36.3%, 34.5%, and 40.2%, respectively. The chloroplast genome contains 131 genes, including 94 protein-coding genes, eight ribosomal RNA genes, and 29 transfer RNA genes. The Maximum-Likelihood Phylogenetic analysis showed a strong sister relationship with F. tataricum in Polygonaceae. Our findings provide a foundation for further investigation of cp genome evolution in F. dibotrys and other higher plants.

