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miR-4319 inhibited retinoblastoma cells proliferation, migration, invasion and EMT progress via suppressing CD147

Zhiqing Wu1, Ling Chen1, Chengyue Zhang2

  • 1Department of Ophthalmology, Xi'an Children's Hospital, No. 69, Xijuyuan Road, Lianhu District, Xi'an, 710003, China.

Journal of Molecular Histology
|January 21, 2021
PubMed

Insights

MicroRNA-4319 (miR-4319) is downregulated in retinoblastoma (RB). Restoring miR-4319 suppresses tumor growth and metastasis by targeting CD147, offering a potential biomarker and therapeutic target for RB.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Tumor migration is a critical factor in retinoblastoma (RB) progression.
  • MicroRNAs (miRNAs) are recognized as key regulators in various cancers, including RB.

Purpose of the Study:

  • To investigate the role of microRNA-4319 (miR-4319) in retinoblastoma development.
  • To identify miR-4319 targets and elucidate its regulatory mechanisms in RB.

Main Methods:

  • Quantitative real-time PCR to assess miR-4319 expression in RB tissues and cell lines.
  • In vitro functional assays (proliferation, migration, invasion, apoptosis) in RB cell lines (SO-RB50, RB-Y79).
  • Bioinformatic analysis and luciferase reporter assays to identify and validate direct targets of miR-4319, focusing on extracellular matrix metalloproteinase inducer (EMMPRI/CD147).

Main Results:

  • miR-4319 expression was significantly downregulated in retinoblastoma tissues and cell lines.
  • Overexpression of miR-4319 inhibited RB cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT), while promoting apoptosis.
  • Extracellular matrix metalloproteinase inducer (CD147) was identified as a direct target of miR-4319, and its suppression affected matrix metalloproteinases (MMPs) expression.
  • Restoration of CD147 expression counteracted the tumor-suppressive effects of miR-4319.

Conclusions:

  • miR-4319 functions as a tumor suppressor in retinoblastoma.
  • miR-4319 inhibits RB cell proliferation, migration, and invasion, potentially by downregulating CD147 and its downstream MMPs.
  • miR-4319 holds promise as a diagnostic biomarker and a therapeutic target for retinoblastoma.
Keywords:
CD417EMTMMPsRetinoblastomamiRNA-4319

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