Closing volume detection by single-breath gas washout and forced oscillation technique

Closing volume (CV) is commonly measured by single-breath nitrogen washout (CV_SBW). A method based on the forced oscillation technique was recently introduced to detect a surrogate CV (CV_FOT). As the two approaches are based on different physiological mechanisms, we aim to investigate CV_FOT and CV_SBW relationship at different degrees and patterns of airway obstruction. A mathematical model was developed to evaluate the CV_SBW and CV_FOT sensitivity to different patterns of airway obstruction, either located in a specific lung region or equally distributed throughout the lung. The two CVs were also assessed during slow vital capacity (VC) maneuvers in triplicate in 13 healthy subjects and pre- and postmethacholine challenge (Mch) in 12 subjects with mild-moderate asthma. Model simulations suggest that CV_SBW is more sensitive than CV_FOT to the presence of few flow-limited or closed airways that modify the contribution of tracer-poor and tracer-rich lung regions to the overall exhaled gas. Conversely, CV_FOT occurs only when at least ∼65% of lung units are flow limited or closed, regardless of their regional distribution. CV_SBW did not differ between healthy subjects and those with asthma (17 ± 9% VC vs. 22 ± 10% VC), whereas CV_FOT did (16 ± 5% VC vs. 23 ± 6% VC, P < 0.01). In patients with asthma, both CV_SBW and CV_FOT increased post-Mch (33 ± 7% VC P < 0.001 and 43 ± 12% VC P < 0.001, respectively). CV_SBW weakly correlated with CV_FOT (r = 0.45, P < 0.01). The closing capacities (CV + residual volume) were correlated (r = 0.74, P < 0.001), but the changes with Mch in both CVs and closing capacities did not correlate. CV_FOT is easy to measure and provides a reproducible parameter useful for describing airway impairment in obstructive respiratory diseases.NEW & NOTEWORTHY The forced oscillation technique can identify a surrogate of closing volume (CV_FOT). We investigated its relationship with the one measured by single-breath washout (CV_SBW). CV_FOT weakly correlates with CV_SBW. The respective closing capacities were correlated, but their increases after methacholine challenge in asthmatics did not. Our results suggest that CV_FOT is less sensitive than CV_SBW to few flow-limited/closed airways but more specific in detecting increases in flow-limited/closed airways involving the majority of the lung.