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Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Related Experiment Video

Updated: Nov 20, 2025

An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function
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High expression of an unknown long noncoding RNA RP11-290L1.3 from GDM macrosomia and its effect on preadipocyte

Yu Lin1, Yingying Zhang1, Lei Xu2

  • 1State Key Laboratory of Reproductive Medicine, Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, People's Republic of China.

Endocrine Connections
|January 21, 2021
PubMed
Summary

Gestational diabetes mellitus (GDM) can cause macrosomia due to increased fat accumulation. Our study shows that the long noncoding RNA RP11 promotes preadipocyte differentiation, potentially explaining fetal fat overgrowth in GDM.

Keywords:
GDMfat accumulationlong noncoding RNAmacrosomiapreadipocyte differentiation

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Area of Science:

  • Endocrinology
  • Molecular Biology
  • Genetics

Background:

  • Gestational diabetes mellitus (GDM)-induced macrosomia is linked to excessive fetal fat accumulation.
  • Adipocyte differentiation plays a key role in this fat accumulation.
  • A long noncoding RNA, RP11, was found upregulated in GDM macrosomia cases.

Purpose of the Study:

  • To investigate the functional role of the long noncoding RNA RP11 in GDM-induced macrosomia.
  • To determine RP11's effect on preadipocyte differentiation and adipogenesis.

Main Methods:

  • Lentivirus-mediated gain- and loss-of-function studies were performed on human visceral preadipocytes (HPA-v).
  • Adipogenesis marker expression (PPARγ, SREBP1c, FASN) was assessed using qPCR and Western blot.
  • Microarray and pathway analysis were employed to elucidate underlying mechanisms.

Main Results:

  • RP11 overexpression significantly enhanced adipocyte differentiation and adipogenesis marker expression.
  • RP11 knockdown demonstrated opposing effects, inhibiting differentiation.
  • Microarray analysis revealed RP11 knockdown affected genes involved in metabolic, insulin signaling, and MAPK signaling pathways.

Conclusions:

  • The long noncoding RNA RP11 acts as a positive regulator of preadipocyte differentiation.
  • RP11's function in promoting adipogenesis may contribute to fetal fat accumulation in GDM.
  • Further research into RP11 could offer insights into managing GDM-induced macrosomia.