Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Photoluminescence: Applications

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Portable Dual-Mode Biosensor for Quantitative Determination of <i>Salmonella</i> in Lateral Flow Assays Using Machine Learning and Smartphone-Assisted Operation.

Biosensors·2026

Same author

The Diamond Age of Cytometry: From Fulwyler to Spectral Multiplexing.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2025

Same author

Enhancing Sensitivity of Commercial Gold Nanoparticle-Based Lateral Flow Assays: A Comparative Study of Colorimetric and Photothermal Approaches.

Sensors (Basel, Switzerland)·2025

Same author

Flow Cytometry: The Next Revolution.

Cells·2023

Same author

Development of an Anti-canine PD-L1 Antibody and Caninized PD-L1 Mouse Model as Translational Research Tools for the Study of Immunotherapy in Humans.

Cancer research communications·2023

Same author

Flow cytometry: past and future.

BioTechniques·2022

Search research articles

Related Experiment Video

Updated: Nov 20, 2025

Optical Trapping of Nanoparticles

Optical Trapping of Nanoparticles

Published on: January 15, 2013

Quantum approach for nanoparticle fluorescence by sub-ns photon detection.

Masanobu Yamamoto^1,2, J Paul Robinson^1,2,3

¹Miftek Corporation, West Lafayette, Indiana, USA.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology

|January 21, 2021

Summary

Detecting tiny nanoparticles like viruses is hard with standard flow cytometry. A new sub-nanosecond sensor and a dry-surface model minimize background noise, improving fluorescence detection for diagnostics.

Keywords:

fluorescence decay nanoparticle fluorescence photo destruction photon-excitation model quantum cytometry reflective dry surface single photon

More Related Videos

Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging

Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging

Published on: July 19, 2016

Compact Quantum Dots for Single-molecule Imaging

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

Related Experiment Videos

Last Updated: Nov 20, 2025

Optical Trapping of Nanoparticles

Optical Trapping of Nanoparticles

Published on: January 15, 2013

Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging

Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging

Published on: July 19, 2016

Compact Quantum Dots for Single-molecule Imaging

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

Area of Science:

Nanotechnology
Biophotonics
Analytical Chemistry

Background:

Conventional flow cytometry struggles with nanoparticle detection due to diffraction limits.
Fluorescence detection of nanoparticles is challenged by low photon counts amidst high background noise.
Water molecular vibrations contribute significantly to background signals in flow cytometry.

Purpose of the Study:

To address challenges in nanoparticle detection using flow cytometry.
To develop a theoretical model for minimizing background noise and enhancing signal.
To investigate nanoparticle-fluorescence analysis parameters and background properties.

Main Methods:

Development of a sub-nanosecond photon sensor exceeding typical fluorescence lifetimes.
Formulation of a theoretical model utilizing a reflective dry surface to minimize water-induced background.
Experimental testing of the model with a 50 nm dried particle on a reflective surface.

Main Results:

The sub-nanosecond sensor enables faster photon detection, crucial for separating individual emitted photons.
The dry-surface model demonstrated potential for background reduction and signal enhancement.
Experimental results with a dried nanoparticle align with established photon-based theory.

Conclusions:

A sub-nanosecond photon sensor is critical for improving nanoparticle detection sensitivity.
A theoretical model using dry surfaces offers a strategy to mitigate background noise in fluorescence analysis.
Further research can optimize parameters for practical nanoparticle-fluorescence analysis and understanding background contributions.