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Related Concept Videos

Chromatographic Methods: Classification01:12

Chromatographic Methods: Classification

3.2K
Chromatographic techniques are classified in three ways: the classification is based on the physical state of the stationary and mobile phases, how the mobile phase and the stationary phase contact each other, or through the chemical or physical processes that isolate the components of the sample. Typically, the mobile phase is either a liquid or gas, while the stationary phase is either a solid or a liquid layer applied to a solid surface.
Chromatographic techniques are typically named by...
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Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

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Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
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Chromatography: Introduction01:10

Chromatography: Introduction

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Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
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Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

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In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
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Affinity Chromatography01:03

Affinity Chromatography

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Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
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High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

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High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
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Chiral chromatography method screening strategies: Past, present and future.

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|January 21, 2021
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Method screening for chiral separations is complex. This review compiles strategies to reduce screening time, aiding laboratories in assessing chiral separation protocols.

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Area of Science:

  • Analytical Chemistry
  • Chromatography
  • Separation Science

Background:

  • Chiral separations are crucial in chromatography but challenging due to complex retention mechanisms.
  • Standard method development involves extensive screening of stationary and mobile phases, often limiting high-throughput analyses.
  • The rate-limiting nature of method screening necessitates efficient strategies for chiral separation development.

Purpose of the Study:

  • To critically review and compile solutions for reducing method-screening time in chiral separations.
  • To provide a comprehensive resource for assessing existing screening protocols in laboratories.
  • To address the challenges in developing predefined method-development steps for diverse chiral molecules.

Main Methods:

  • Literature review of solutions published over the last two decades.
  • Critical discussion and compilation of diverse method-screening strategies.
  • Analysis of adopted and confined strategies in chiral separation science.

Main Results:

  • Identification of various strategies proposed to reduce net method-screening time.
  • Assessment of the practical adoption and theoretical confinement of these strategies.
  • Compilation of solutions to aid laboratories in evaluating their chiral separation protocols.

Conclusions:

  • A consolidated review of method-screening strategies can significantly aid laboratories in chiral separations.
  • Effective screening protocols are essential for overcoming the complexity of chiral stationary-phase retention.
  • This review provides a valuable resource for optimizing high-throughput analyses and purification workflows in enantiomeric separations.